Detection of protein interactions based on GFP fragment complementation by fluorescence microscopy and spectrofluorometry
نویسندگان
چکیده
منابع مشابه
Detection of protein interactions based on GFP fragment complementation by fluorescence microscopy and spectrofluorometry.
We have developed a set of simple modifications of the green fluorescent protein (GFP)fragment reassembly assay in bacteria that permits: (i)fluorescent microscopy visualization of GFP reassembly only 1-2 h after induction of protein expression, thus approximating the detection of GFP reassembly to the real-time dynamics of protein complex formation in living cells; (ii) spectrofluorometric det...
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متن کاملThe analysis of protein-protein interactions in plants by bimolecular fluorescence complementation.
Following the complete genome sequencing of different plant species such as Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), and Physcomitrella (Physcomitrella patens), as well as advances toward deciphering entire proteomes, the need for a reliable way to identify protein-protein interactions is becoming a major task for the future. Bimolecular fluorescent complementation (BiFC) is a n...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2008
ISSN: 0736-6205,1940-9818
DOI: 10.2144/000112685